Pretreatment of date syrup to increase citric acid production

Pretreatment of date syrup with sulfuric acid, tricalcium phosphate, tricalcium phosphate with hydrochloric acid, potassium ferrocyanide, and EDTA to increase the production of citric acid was investigated. Among the above techniques used for the removal of heavy metals, 2% tricalcium phosphate trea...

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Bibliographic Details
Published in:Enzyme and microbial technology 1997-09, Vol.21 (4), p.273-276
Main Authors: Roukas, T., Kotzekidou, P.
Format: Article
Language:English
Subjects:
Aspergillus niger
Biological and medical sciences
Biotechnology
Citric acid
date syrup
Fundamental and applied biological sciences. Psychology
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
surface fermentation
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Summary:Pretreatment of date syrup with sulfuric acid, tricalcium phosphate, tricalcium phosphate with hydrochloric acid, potassium ferrocyanide, and EDTA to increase the production of citric acid was investigated. Among the above techniques used for the removal of heavy metals, 2% tricalcium phosphate treatment gave better results regarding citric acid concentration (55 ± 1.5 g l −1), citric acid yield (50 ± 1.5%), and sugar utilization (73.3 ± 1%). The optimum pH for citric acid production was 6.5. The addition of 4% (ν/ν) methanol in the date syrup solution treated with 2% tricalcium phosphate increased the concentration of citric acid from 55 to 90 g l −1.
ISSN:0141-0229
1879-0909
DOI:10.1016/S0141-0229(97)00041-0