Enzymatic production of N-acetyl- d-glucosamine from chitin. Degradation study of N-acetylchitooligosaccharide and the effect of mixing of crude enzymes

N-Acetyl- d-glucosamine (GlcNAc) was produced from chitin by use of crude enzyme preparations. The efficient production of GlcNAc by cellulases derived from Trichoderma viride (T) and Acremonium cellulolyticus (A) was observed by HPLC analysis compared to lipase, hemicellulase, and pectinase. β-Chit...

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Published in:Carbohydrate polymers 2003-03, Vol.51 (4), p.391-395
Main Authors: Sashiwa, Hitoshi, Fujishima, Shizu, Yamano, Naoko, Kawasaki, Norioki, Nakayama, Atsuyoshi, Muraki, Einosuke, Sukwattanasinitt, Mongkol, Pichyangkura, Rath, Aiba, Sei-ichi
Format: Article
Language:English
Subjects:
Applied sciences
Chitin
Crude enzyme
Exact sciences and technology
N-Acetyl- d-glucosamine
Natural polymers
Physicochemistry of polymers
Starch and polysaccharides
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Summary:N-Acetyl- d-glucosamine (GlcNAc) was produced from chitin by use of crude enzyme preparations. The efficient production of GlcNAc by cellulases derived from Trichoderma viride (T) and Acremonium cellulolyticus (A) was observed by HPLC analysis compared to lipase, hemicellulase, and pectinase. β-Chitin showed higher degradability than α-chitin when using cellulase T. The optimum pH of cellulase T was 4.0 on the hydrolysis of β-chitin. The yield of GlcNAc was enhanced by mixing of cellulase T and A.
ISSN:0144-8617
1879-1344
DOI:10.1016/S0144-8617(02)00192-3