DNA-binding and photocleavage studies of cobalt(III) polypyridyl complexes: [Co(phen) 2IP] 3+ and [Co(phen) 2PIP] 3

Two complexes of [Co(phen) 2IP] 3+ (IP=imidazo[4,5- f][1,10]phenanthroline) and [Co(phen) 2PIP] 3+ (PIP=2-phenylimidazo[4,5- f][1,10]phenanthroline) have been synthesized and characterized by UV/VIS, IR, EA and mass spectra. The binding of the two complexes with calf thymus DNA has been investigated...

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Bibliographic Details
Published in:Journal of inorganic biochemistry 2001, Vol.83 (1), p.49-55
Main Authors: Zhang, Qian-Ling, Liu, Jin-Gang, Chao, Hui, Xue, Gen-Qiang, Ji, Liang-Nian
Format: Article
Language:English
Subjects:
Cobalt(III) complexes
DNA-binding
Photocleavage
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Summary:Two complexes of [Co(phen) 2IP] 3+ (IP=imidazo[4,5- f][1,10]phenanthroline) and [Co(phen) 2PIP] 3+ (PIP=2-phenylimidazo[4,5- f][1,10]phenanthroline) have been synthesized and characterized by UV/VIS, IR, EA and mass spectra. The binding of the two complexes with calf thymus DNA has been investigated by absorption spectroscopy, cyclic voltammetry, viscosity measurements and DNA cleavage assay. The spectroscopic studies together with cyclic voltammetry and viscosity experiments support that both of the complexes bind to CT DNA by intercalation via IP or PIP into the base pairs of DNA. [Co(phen) 2PIP] 3+ binds more avidly to CT DNA than [Co(phen) 2IP] 3+, which is consistent with the extended planar and π system of PIP. Noticeably, the two complexes have been found to be efficient photosensitisers for strand scissions in plasmid DNA.
ISSN:0162-0134
1873-3344
DOI:10.1016/S0162-0134(00)00132-X