Changes in some characteristics between the wild and Al-tolerant coffee ( Coffea arabica L.) cell line

• Published in: Journal of inorganic biochemistry 2003-09, Vol.97 (1), p.69-78
• Main Author: MARTINEZESTEVEZ, M
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological - drug effects; Adaptation, Physiological - physiology; Alleles; Aluminum - analysis; Aluminum - chemistry; Aluminum - metabolism; Aluminum - toxicity; Aluminum tolerance; Cell Line; Cell Membrane - drug effects; Cell Membrane - metabolism; Coffee - cytology; Coffee - drug effects; Coffee - genetics; Coffee - metabolism; Coffee cells; DNA Primers - genetics; Fluorescence; Hydrogen-Ion Concentration; Microsatellite Repeats - genetics; Osmolar Concentration; Signal transduction; Spectrophotometry, Atomic; Type C Phospholipases - metabolism
• ISSN: 0162-0134; 1873-3344
• DOI: 10.1016/S0162-0134(03)00260-5

An aluminium (Al)-tolerant cell line (LAMt) of coffee ( Coffea arabica L.) was obtained from a cell suspension culture and biochemically and molecularly characterized in an MS medium at half ionic strength and low pH. LAMt grew 30% more than the control line (susceptible to Al) in the presence of different concentrations of Al, showed a lower free Al concentration in the medium and had higher phospholipase C specific activity (80%). Membrane integrity of the LAMt was 50% greater than the control line when both were incubated in the presence of different Al concentrations (measured by Evans Blue uptake). Finally, the use of microsatellite primers revealed no difference in the DNA pattern of both cell lines.