Pyrolysis–gas chromatography/mass spectrometry of peroxynitrite-treated melanins

• Published in: Journal of analytical and applied pyrolysis 2003-12, Vol.70 (2), p.457-467
• Main Authors: Dzierżęga-Lęcznar, Anna, Stępień, Krystyna, Chodurek, Ewa, Kurkiewicz, Sławomir, Świątkowska, Longina, Wilczok, Tadeusz
• Format: Article
• Language: English
• Subjects: CysDA-melanin; DA-melanin; Neuromelanin; Peroxynitrite; Pyrolysis–gas chromatography/mass spectrometry
• ISSN: 0165-2370; 1873-250X
• DOI: 10.1016/S0165-2370(03)00004-4

Peroxynitrite—a strong oxidizing and nitrating agent—is thought to be a mediator of the cytotoxic action of nitric oxide in biological systems. There is some evidence that peroxynitrite-dependent processes may be involved in the pathomechanism of Parkinson's disease. Recently, it has been suggested that neuromelanin, present in the cytoplasm of nigrostriatal neurons, can act as natural scavenger of peroxynitrite. In this study the effect of peroxynitrite on the structure of synthetic models of human neuromelanin was investigated. Model neuromelanins, obtained from dopamine (DA) and/or 5- S-cysteinyldopamine (5- S-CysDA), were pyrolyzed at 770 °C before and after treatment with peroxynitrite and the thermal degradation products separated by gas chromatography were identified by mass spectrometry. After exposure to peroxynitrite, reduced content of the typical pyrolysis products accompanied by elevated levels of low molecular weight gases in the pyrolysates were observed. The changes in pyrolytic patterns were dependent on melanin type and peroxynitrite concentration. It was concluded that interaction with peroxynitrite causes extensive oxidative degradation of the analyzed pigments. This may be of great biological importance with regard to depigmentation of nigrostriatal neurons demonstrated in Parkinson's disease.