Endothelin-1 induces contraction of human and Australian possum gallbladder in vitro

Background: Endothelin-1 (ET-1) stimulates guinea pig gallbladder (GB) muscle strip contractility; however, the role and source of ET-1 in the GB remains to be elucidated. Aims: To determine the effect of ET-1 on human and possum GB muscle strip contractility and evaluate whether ET-1 is present in...

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Bibliographic Details
Published in:Regulatory peptides 2001-10, Vol.102 (1), p.31-39
Main Authors: Al-Jiffry, Bilal O, Meedeniya, Adrian C.B, Chen, John W, Toouli, James, Saccone, Gino T.P
Format: Article
Language:English
Subjects:
Animals
Australia
Biological and medical sciences
Dose-Response Relationship, Drug
Drug Combinations
Endothelin antagonists
Endothelin Receptor Antagonists
Endothelin-1 - pharmacology
Fundamental and applied biological sciences. Psychology
Gallbladder - cytology
Gallbladder - drug effects
Gallbladder - physiology
Gallbladder motility
Humans
Immunohistochemistry
In Vitro Techniques
Liver. Bile. Biliary tracts
Muscle Contraction - drug effects
Oligopeptides - pharmacology
Opossums
Peptides, Cyclic - pharmacology
Piperidines - pharmacology
Tetrodotoxin - pharmacology
Vertebrates: digestive system
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Summary:Background: Endothelin-1 (ET-1) stimulates guinea pig gallbladder (GB) muscle strip contractility; however, the role and source of ET-1 in the GB remains to be elucidated. Aims: To determine the effect of ET-1 on human and possum GB muscle strip contractility and evaluate whether ET-1 is present in GB tissue. Methods: GB muscle strips were mounted in organ baths to measure isometric tension. ET-1 was added cumulatively with and without pretreatment with the neural blocker tetrodotoxin (TTX) or the ET receptor antagonists BQ-123, BQ-788, and tezosentan. Immunohistochemical localization of ET was performed on freshly fixed and cultured GBs. Results: ET-1 induced concentration-dependent increases in tone in human and possum GB strips ( p
ISSN:0167-0115
1873-1686
DOI:10.1016/S0167-0115(01)00302-0