Transcriptional activation of the alternative oxidase gene of the fungus Magnaporthe grisea by a respiratory-inhibiting fungicide and hydrogen peroxide

Alternative oxidase (AOX) is dramatically induced when the fungus Magnaporthe grisea is incubated with the fungicide SSF-126, which interacts with the cytochrome bc 1 complex in the electron transport system of mitochondria. A full-length cDNA for the alternative oxidase gene ( AOX) was obtained, an...

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Bibliographic Details
Published in:Biochimica et biophysica acta 1998-11, Vol.1442 (2), p.161-169
Main Authors: Yukioka, Hideo, Inagaki, Shuichiro, Tanaka, Reiji, Katoh, Kenji, Miki, Nobuo, Mizutani, Akira, Masuko, Michio
Format: Article
Language:English
Subjects:
Active oxygen species
Alternative oxidase
Amino Acid Sequence
amino acid sequences
Arabidopsis - enzymology
Cloning, Molecular
complementary DNA
Conserved Sequence
Cyanide-resistant respiration
Cycloheximide - pharmacology
electron transfer
Electron transport
Electron Transport - drug effects
Electron Transport Complex III - drug effects
Electron Transport Complex III - metabolism
Fungicides, Industrial - pharmacology
genbank/ab005144
Gene Expression Regulation, Enzymologic - drug effects
Gene Expression Regulation, Fungal - drug effects
Hydrogen Peroxide - pharmacology
Magnaporthe - drug effects
Magnaporthe - enzymology
Magnaporthe - genetics
Mitochondria - drug effects
Mitochondria - metabolism
Mitochondrial Proteins
Molecular Sequence Data
nucleotide sequences
oxidoreductases
Oxidoreductases - biosynthesis
Oxidoreductases - genetics
Oxygen Consumption
Plant Proteins
Polymerase Chain Reaction
Protein Biosynthesis
respiration
Sequence Alignment
Sequence Homology, Amino Acid
Superinduction
Transcription
transcriptional activation
Transcriptional Activation - drug effects
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Summary:Alternative oxidase (AOX) is dramatically induced when the fungus Magnaporthe grisea is incubated with the fungicide SSF-126, which interacts with the cytochrome bc 1 complex in the electron transport system of mitochondria. A full-length cDNA for the alternative oxidase gene ( AOX) was obtained, and the deduced amino acid sequence revealed marked similarity to other AOXs, but lacks two cysteine residues at corresponding sites which are conserved in plant AOXs and play essential roles in the post-translational regulation. Northern blot experiments showed that treatment of M. grisea cells with SSF-126 induces accumulation of AOX mRNA in a dose-dependent manner, and the level was correlated with the activity of alternative respiration. H 2O 2 also induced the accumulation of the transcript with a short half-life (
ISSN:0167-4781
0006-3002
1879-2634
1878-2434
DOI:10.1016/S0167-4781(98)00159-6