Characterization of a cDNA homologous to carotenoid-associated protein in citrus fruits

• Published in: Biochimica et biophysica acta 1998-11, Vol.1442 (2), p.334-338
• Main Authors: Moriguchi, Takaya, Kita, Masayuki, Endo-Inagaki, Tomoko, Ikoma, Yoshinori, Omura, Mitsuo
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; amino acid sequences; Base Sequence; Carotenoid-associated protein; Carrier Proteins - biosynthesis; Carrier Proteins - chemistry; Carrier Proteins - genetics; Citrus - genetics; Citrus aurantium var. chrysocarpa; Citrus unshiu; complementary DNA; DNA, Complementary; Fruit development; genbank/ab011797; Molecular Sequence Data; nucleotide sequences; Plant Leaves; Plant Proteins - genetics; Plastid-lipid-associated protein; Recombinant Proteins - biosynthesis; Recombinant Proteins - chemistry; Satsuma mandarin; Sequence Alignment; Sequence Homology, Amino Acid
• ISSN: 0167-4781; 0006-3002; 1879-2634; 1878-2434
• DOI: 10.1016/S0167-4781(98)00182-1

A cDNA (CitPAP) homologous to a gene encoding for Cucumis sativus carotenoid-associated protein (CHRC) has been isolated from satsuma mandarin ( Citrus unshiu Marc.). Unlike ChrC whose expression was limited only in mature fruits (containing chromoplasts), CitPAP transcripts were detected in all the tissues examined including fruits, flowers and leaves. In this respect, CitPAP was rather close to a gene encoding for pepper plastid-lipid-associated protein (PAP), which exhibits ubiquitous expression in bell pepper organs containing chloroplasts or chromoplasts. CitPAP, however, differed from PAP in the magnitude and pattern of RNA accumulation. These results might indicate a novel function of CitPAP.