Structure, expression profile and chromosomal location of an isolog of DNA-PKcs interacting protein (KIP) gene

• Published in: Biochimica et biophysica acta 1999-01, Vol.1444 (1), p.143-147
• Main Authors: Seki, Naohiko, Hattori, Astushi, Hayashi, Akiko, Kozuma, Sumie, Ohira, Miki, Hori, Tada-aki, Saito, Toshiyuki
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Base Sequence; Brain - embryology; Brain - metabolism; Calcineurin - chemistry; Calcium-Binding Proteins - genetics; Chromosome Mapping; Cyclin-Dependent Kinase Inhibitor p57; DNA, Complementary - isolation & purification; Gene Library; Humans; Molecular Sequence Data; Nuclear Proteins - biosynthesis; Nuclear Proteins - chemistry; Nuclear Proteins - genetics; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - biosynthesis; Sequence Alignment; Sequence Homology, Amino Acid
• ISSN: 0167-4781; 0006-3002; 1879-2634
• DOI: 10.1016/S0167-4781(98)00253-X

A novel DNA-PKcs interacting protein, KIP (kinase interacting protein), was recently isolated using a two-hybrid analysis which showed a significant homology to calcineurin B. We found other ESTs showing significant similarity to KIP gene in the dbEST database and isolated a cDNA clone which encodes a 187 amino acid polypeptide from a human fetal brain cDNA library. This protein (termed KIP2 for kinase interacting protein 2) has sequence homology to KIP (46% identical and 64% similarity). RT-PCR analysis showed that the messenger RNA was ubiquitously expressed in various human tissues. Based on PCR-based analysis with a radiation hybrid cell panel and fluorescence in situ hybridization, the gene was localized to the q24 region of chromosome 15.