Proton-NMR investigation of the heme cavity in the cyanomet derivative of the cooperative homodimeric hemoglobin from Scapharca inaequivalvis

• Published in: Biochimica et biophysica acta 1996-12, Vol.1298 (2), p.261-275
• Main Authors: Wu, Yuyang, Basti, Mufeed, Gambacurta, Alessandra, Chiancone, Emilia, Ascoli, Franca, La Mar, Gerd N.
• Format: Article
• Language: English
• Subjects: Animals; Bivalvia; Crystallography, X-Ray; Dimeric hemoglobin; Heme - chemistry; Heme cavity; Hemoglobins - chemistry; Hyperfine shift; Magnetic axes; Magnetic Resonance Spectroscopy; NMR; Protein Conformation; Recombinant Proteins - chemistry; Scapharca inaequivalvis
• ISSN: 0167-4838; 0006-3002; 1879-2588
• DOI: 10.1016/S0167-4838(96)00137-9

The active-site structure of the paramagnetic cyanomet complex of the cooperative homodimeric hemoglobin from Scapharca inaequivalvis has been investigated by solution homonuclear NMR. In spite of the large size (32 kDa), the residues on the key proximal F- and distal E-helices could be sequence-specifically assigned and placed in the heme pocket in a manner common to diamagnetic systems. These backbone assignments were greatly facilitated by the significant dispersion of backbone chemical shifts by the highly anisotropic paramagnetic susceptibility tensor of the low-spin ferric state. The remainder of the residues in contact with the heme are assigned based on unique contacts to the heme predicted by the crystal structure and the observations of scalar connectivities diagnostic for the residues. The magnitude of the dipolar shifts for non-ligated residues was used to determine the anisotropy and orientation of the paramagnetic susceptibilty tensor, and the major axis found tilted from the normal in a manner similar to that found for the FeCO unit in the crystal structure. The combination of NOESY inter-residue and heme-residue contacts, paramagnetic-induced relaxation and correlation between observed and dipolar shifts provide a description of the heme cavity in cyanomet Hb that is essentially the same as found in the carbonmonoxy Hb crystal structure. The pattern of both the heme methyl dominant contact shifts and the heme meso-proton dominant dipolar shifts are shown to be consistent with the orientation of the axial His. It is concluded that the present homonuclear NMR methods allow effective solution structure determination in the cyanomet form for dimeric Hb and suggest profitable extension to the tetrameric vertebrate hemoglobins.