Influence of freeze-concentration effect on proteinase-catalysed peptide synthesis in frozen aqueous systems

Freezing of the reaction mixture is a powerful tool in proteinase-catalysed peptide synthesis. In this study, the considerable yield-increasing effect of freezing has been analysed by physical and analytical methods. 1H-NMR relaxation time measurements have been used to determine the amount of unfro...

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Bibliographic Details
Published in:Biochimica et biophysica acta 1997-04, Vol.1338 (2), p.253-258
Main Authors: Ullmann, G, Haensler, M, Gruender, W, Wagner, M, Hofmann, H.-J, Jakubke, H.-D
Format: Article
Language:English
Subjects:
Catalysis
Chemical Phenomena
Chemistry, Physical
Chymotrypsin - metabolism
Cysteine Endopeptidases - metabolism
Endopeptidases - metabolism
Enzymatic peptide synthesis
Freeze-concentration effect
Freezing
Frozen aqueous system
Kinetics
Magnetic Resonance Spectroscopy
NMR relaxation
Papain - metabolism
Peptide Biosynthesis
Peptide synthesis
Proteinase
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Summary:Freezing of the reaction mixture is a powerful tool in proteinase-catalysed peptide synthesis. In this study, the considerable yield-increasing effect of freezing has been analysed by physical and analytical methods. 1H-NMR relaxation time measurements have been used to determine the amount of unfrozen water in partially frozen systems thus quantifying the extent of the `freeze concentration effect' for the first time. Comparative studies in ice and at room temperature verify the importance of freeze-concentration which, however, is not sufficient for a complete understanding of the observed effects. Furthermore, the phase behaviour of frozen systems is discussed. © 1997 Elsevier Science B.V.
ISSN:0167-4838
0006-3002
1879-2588
DOI:10.1016/S0167-4838(96)00207-5