cDNA cloning of bovine thrombospondin 1 and its expression in odontoblasts and predentin

The extracellular matrix protein thrombospondin 1 (TSP1) was cloned from odontoblasts of bovine mandibular teeth which participate in dentinogenesis. The 5289 bp cDNA contains a complete open reading frame of 1170 amino acids. Bovine TSP1 has high homologies to its human and mouse counterparts. In i...

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Bibliographic Details
Published in:Biochimica et biophysica acta 1998-01, Vol.1382 (1), p.17-22
Main Authors: Ueno, Akemichi, Yamashita, Kikuji, Nagata, Toshihiko, Tsurumi, Chizuko, Miwa, Yoshihiro, Kitamura, Seiichiro, Inoue, Hideo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Bos taurus
Cattle
Chickens
Cloning, Molecular
Dentinogenesis
DNA, Complementary
Humans
Mice
Molecular Sequence Data
Odontoblast
Odontoblasts - metabolism
Predentin
Rats
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Sequence Alignment
Sequence Homology, Amino Acid
Thrombospondin
Thrombospondin 1 - biosynthesis
Thrombospondin 1 - chemistry
Xenopus
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Summary:The extracellular matrix protein thrombospondin 1 (TSP1) was cloned from odontoblasts of bovine mandibular teeth which participate in dentinogenesis. The 5289 bp cDNA contains a complete open reading frame of 1170 amino acids. Bovine TSP1 has high homologies to its human and mouse counterparts. In immunohistochemical analyses of bovine anterior teeth with anti-TSP1 monoclonal antibody, TSP1 was only detectable at the position of predentin, located between dentin and unmineralized dental pulp. Northern blot analysis showed high levels of two sizes of TSP1 mRNAs in odontoblasts but not dental pulp and gingiva. Previously we found that osteotropic factors such as calcitriol and TGF-β induce TSP1 at the transcriptional level in clonal rat dental pulp cells. These results suggest a role of TSP1 in dentinogenesis and/or maintenance of dentin and dental pulp.
ISSN:0167-4838
0006-3002
1879-2588
DOI:10.1016/S0167-4838(97)00188-X