Effect of ionic strength on the interfacial properties of cytochrome c

The surface tension behaviour of oxidised cytochrome c (cyt c) solution was characterised at various pH and ionic strength at the air/water interface. The pendant drop method employing digital image analysis of the drop shape was applied to the measurement of the surface tension. The adsorption prop...

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Bibliographic Details
Published in:Biochimica et biophysica acta 1999-06, Vol.1432 (1), p.82-91
Main Authors: Gažová, Zuzana, Antalı́k, Marián, Bágel’ová, Jaroslava, Tomori, Zoltán
Format: Article
Language:English
Subjects:
Cytochrome c Group - chemistry
Ferricytochrome c
Hydrogen-Ion Concentration
Molten globule state
Osmolar Concentration
Oxidation-Reduction
Pendant drop method
Protein Conformation
Solutions
Surface Tension
Unfolding
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Summary:The surface tension behaviour of oxidised cytochrome c (cyt c) solution was characterised at various pH and ionic strength at the air/water interface. The pendant drop method employing digital image analysis of the drop shape was applied to the measurement of the surface tension. The adsorption properties of cyt c were utilised to study the protein conformation change effected by acidification and ionic strength. At high ionic strength, the saturated steady-state surface tension shows a cooperative change centred around 3.6 induced by a decrease in pH. Using spectroscopic experiments, the apparent p K of the acid-induced transition of horse cyt c from the native to the molten globular state is equal to 3.5. This fact indicates that the saturated steady-state surface tension is a parameter which might be used to monitor conformation changes of cyt c.
ISSN:0167-4838
0006-3002
1879-2588
DOI:10.1016/S0167-4838(99)00091-6