Isolation of a Dictyostelium discoideum 14-3-3 homologue

• Published in: Biochimica et biophysica acta 1997-06, Vol.1357 (2), p.243-248
• Main Authors: Knetsch, Menno L.W, van Heusden, G.Paul H, Ennis, Herbert L, Shaw, David R, Epskamp, Stefan J.P, Snaar-Jagalska, B.Ewa
• Format: Article
• Language: English
• Subjects: 14-3-3; 14-3-3 Proteins; Amino Acid Sequence; Animals; Base Sequence; Blotting, Southern; Blotting, Western; Cellular slime mold; Developmental regulation; Dictyostelium - genetics; Dictyostelium - growth & development; DNA, Complementary - chemistry; DNA, Complementary - isolation & purification; Ftta locus; Functional complementation; Gene Expression; Hordeum - genetics; Molecular Sequence Data; Polymerase Chain Reaction; Proteins - chemistry; Proteins - genetics; RNA, Messenger - metabolism; Saccharomyces cerevisiae - genetics; Sequence Homology; Tyrosine 3-Monooxygenase
• ISSN: 0167-4889; 0006-3002; 1879-2596
• DOI: 10.1016/S0167-4889(97)00060-8

A 1.0 kb cDNA clone ( Dd14- 3- 3) encoding a 14-3-3 homologue was isolated from a Dictyostelium discoideum cDNA library. The putative Dd14-3-3 protein has highest sequence identity to a barley 14-3-3 isoform (74%). Southern blot analysis suggests that only one 14-3-3 gene is present in the Dictyostelium genome. Highest Dd14- 3- 3 expression is observed in vegetatively growing cells, and expression decreases during multicellular development. In contrast, Dd14-3-3 protein levels detected immunochemically remained constant during Dictyostelium development. Expression of the Dd14- 3- 3 cDNA in Saccharomyces cerevisiae complemented the lethal disruption of the two yeast genes encoding 14-3-3 proteins ( BMH1 and BMH2). This shows that Dd14-3-3 can fulfil the same function(s) as the yeast 14-3-3 proteins.