Tobacco BY-2 cell-free extracts induce the recovery of microtubule nucleating activity of inactivated mammalian centrosomes

The structure and the molecular composition of the microtubule-organizing centers in acentriolar higher plant cells remain unknown. We developed an in vitro complementation assay where tobacco BY-2 extracts can restore the microtubule-nucleating activity of urea-inactivated mammalian centrosomes. Ou...

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Bibliographic Details
Published in:Biochimica et biophysica acta 1999-02, Vol.1449 (1), p.101-106
Main Authors: Stoppin-Mellet, Virginie, Peter, Christine, Buendia, Brigitte, Karsenti, Eric, Lambert, Anne-Marie
Format: Article
Language:English
Subjects:
Centrosome
Centrosome - chemistry
Centrosome - drug effects
Centrosome - physiology
Higher plants
Microtubule
Microtubule-organizing center
Microtubules - physiology
Nicotiana - genetics
Nicotiana - metabolism
Nicotiana - ultrastructure
Nucleation
plant biochemistry
Plant Extracts - pharmacology
plant physiology
Plants, Toxic
Urea
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Summary:The structure and the molecular composition of the microtubule-organizing centers in acentriolar higher plant cells remain unknown. We developed an in vitro complementation assay where tobacco BY-2 extracts can restore the microtubule-nucleating activity of urea-inactivated mammalian centrosomes. Our results provide first evidence that soluble microtubule-nucleating factors are present in the plant cytosolic fraction. The implication for microtubule nucleation in higher plants is discussed.
ISSN:0167-4889
0006-3002
1879-2596
1878-2434
DOI:10.1016/S0167-4889(98)00179-7