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Isolation of acetone/ethylene glycol utilizing and biofilm forming strains of bacteria

Packed columns of zeolite A-Ca particles, fed with acetone or/and ethylene glycol containing synthetic medium, provide a tool for a physiological preparation of bacterial cell populations with solvent-utilizing and biofilm forming activity. Phased procedure and outlined schemes of column operations...

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Bibliographic Details
Published in:Journal of microbiological methods 1997-08, Vol.30 (2), p.133-139
Main Authors: MASAK, J, CEJKOVA, A, JIRKU, V
Format: Article
Language:English
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Summary:Packed columns of zeolite A-Ca particles, fed with acetone or/and ethylene glycol containing synthetic medium, provide a tool for a physiological preparation of bacterial cell populations with solvent-utilizing and biofilm forming activity. Phased procedure and outlined schemes of column operations enable one to perform (under required conditions) high yield strain isolations and long-term (easy to monitor) physiological adaptations of both activities. Regarding attempts to isolate strains in the presence of a specific xenobiotic compound, the procedure is ready to be tested/applied across a wider spectrum of xenobiotics.
ISSN:0167-7012
1872-8359
DOI:10.1016/S0167-7012(97)00055-9