Enzyme reactions in the presence of cyclodextrins: biosensors and enzyme assays

Cyclodextrins, macrocyclic carbohydrates with apolar internal cavities, can form complexes with, and solubilize many normally water-insoluble compounds. Ferrocene and its derivaties, tetrathiafulvalene and tetramethylbenzidine, can function as redox mediators, but are insoluble in water; when they a...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 1995-11, Vol.13 (11), p.457-463
Main Authors: Luong, John H.T., Brown, R. Stephen, Male, Keith B., Cattaneo, Maurice V., Zhao, Shishan
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biosensing Techniques
Biosensors
Biotechnology
Cyclodextrins - pharmacology
Environmental Monitoring
Enzymes - analysis
Fundamental and applied biological sciences. Psychology
Humans
Methods. Procedures. Technologies
Solubility
Various methods and equipments
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Summary:Cyclodextrins, macrocyclic carbohydrates with apolar internal cavities, can form complexes with, and solubilize many normally water-insoluble compounds. Ferrocene and its derivaties, tetrathiafulvalene and tetramethylbenzidine, can function as redox mediators, but are insoluble in water; when they are complexed with cyclodextrins, they can be used in enzymatic assays and in the construction of mediated biosensors. In addition, the solubilization of polynuclear aromatic hydrocarbons (PAHs), including the potent carcinogen benzo[a]pyrene, by cyclodextrins has enabled the detection of these important environmental contaminants.
ISSN:0167-7799
1879-3096
DOI:10.1016/S0167-7799(00)89002-2