Subnuclear distribution of afferents from the oral, pharyngeal and laryngeal regions in the nucleus tractus solitarii of the rat: a study using transganglionic transport of cholera toxin

• Published in: Neuroscience research 2001-02, Vol.39 (2), p.221-232
• Main Authors: Hayakawa, Tetsu, Takanaga, Akinori, Maeda, Seishi, Seki, Makoto, Yajima, Yukio
• Format: Article
• Language: English
• Subjects: Afferent Pathways - anatomy & histology; Afferent Pathways - cytology; Afferent Pathways - physiology; Animals; Anterograde tracing; Axonal Transport; Brain Mapping; Cholera Toxin - pharmacokinetics; Gustatory inputs; Laryngeal Nerves - anatomy & histology; Laryngeal Nerves - cytology; Laryngeal Nerves - physiology; Male; Mouth - innervation; Pharynx - innervation; Rats; Rats, Sprague-Dawley; Solitary Nucleus - anatomy & histology; Solitary Nucleus - cytology; Solitary Nucleus - physiology; Somatosensory; Swallowing; Viscerosensory
• ISSN: 0168-0102; 1872-8111
• DOI: 10.1016/S0168-0102(00)00218-2

The central distributions of afferents from the oral cavity, the pharynx, the larynx and the esophagus to the nucleus tractus solitarii (NTS) were examined by using transganglionic anterograde transport of the cholera toxin B subunit (CT-b). Injections of CT-b into the body of the tongue and the hard palate resulted in heavy labeling of the lateral subnucleus (l-NTS) of the NTS rostral to the area postrema. Injection into the root of the tongue resulted in heavy labeling of the l-NTS, the dorsal half of the medial (m-NTS), the intermediate (im-NTS) and the interstitial (is-NTS) subnuclei rostral to the area postrema. Injections into the soft palate and the pharynx resulted in a similar labeling pattern in the is-NTS, im-NTS and m-NTS to that in the case of the root of the tongue, but this labeling extended rostrocaudally. Heavy labeling of the medial aspect of the l-NTS was found in the case of the soft palate, but the labeling was sparse in the case of the pharynx. Moderate labeling was also found in the commissural subnucleus (co-NTS). Injection into the larynx resulted in labeling of the is-NTS throughout the NTS, and of the rostral half of im-NTS. Injection into the esophagus resulted in heavy labeling of the central subnucleus, and moderate labeling of the co-NTS and the caudal half of im-NTS. A few but consistent anterogradely labeled terminals were found to appose retrogradely labeled small neurons in the rostral tip of the dorsal motor nucleus of vagus in the cases of injections into the root of the tongue, the soft palate, the pharynx, and the larynx. These results have characterized the viscerotopic representation of afferent projections from the oral and the cervical visceral organs to the subnuclei of the NTS.