Nicotine increases cytosolic Ca 2+in vasopressin neurons

Strong immunoreactivity for neuronal nicotinic acetylcholine receptor α4 subunit was detected in neurons of the supraoptic nucleus (SON). At the ultrastructural level, immunoreactivity for α4 was detected in the post-synaptic membranes as well as in the cytoplasmic matrices in the magnocellular neur...

Full description

Saved in:
Bibliographic Details
Published in:Neuroscience research 1997-12, Vol.29 (4), p.311-318
Main Authors: Shioda, Seiji, Yada, Toshihiko, Muroya, Shinji, Takigawa, Morikuni, Nakai, Yasumitsu
Format: Article
Language:English
Subjects:
Acetylcholine receptor
cAMP
Cytosolic Ca 2
In vitro study
Nicotine
Rat
Vasopressin
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Strong immunoreactivity for neuronal nicotinic acetylcholine receptor α4 subunit was detected in neurons of the supraoptic nucleus (SON). At the ultrastructural level, immunoreactivity for α4 was detected in the post-synaptic membranes as well as in the cytoplasmic matrices in the magnocellular neurons. Nicotine (1–10 μM) increased cytosolic Ca 2+concentrations ([Ca 2+] i) in isolated arginine-vasopressin (AVP)-containing neurons in the rat SON. Nicotine (10 μM) was less potent in increasing [Ca 2+] i in AVP-containing neurons than noradrenaline (1 μM), a known neurotransmitter in the SON. The nicotine-induced [Ca 2+] i increase in AVP-containing neurons was markedly reduced when pre-treated with a protein kinase A (PKA) blocker, H89 (40 μM). These findings suggest that nicotine, a known neurotransmitter in the SON, activates AVP-containing neurons via nicotinic acetylcholine receptor which is linked to stimulation of cAMP-PKA-regulated Ca 2+signaling pathway.
ISSN:0168-0102
1872-8111
DOI:10.1016/S0168-0102(97)00103-X