High resolution ion mobility measurements for gas phase proteins: correlation between solution phase and gas phase conformations

Our high resolution ion mobility apparatus has been modified by attaching an electrospray source to perform measurements for biological molecules. While the greater resolving power permits the resolution of more conformations for BPTI and cytochrome c, the resolved features are generally much broade...

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Bibliographic Details
Published in:International journal of mass spectrometry and ion processes 1997-11, Vol.165, p.497-507
Main Authors: Hudgins, Robert R., Woenckhaus, Jürgen, Jarrold, Martin F.
Format: Article
Language:English
Subjects:
Cytochrome c
Electrospray
Protein conformation
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Summary:Our high resolution ion mobility apparatus has been modified by attaching an electrospray source to perform measurements for biological molecules. While the greater resolving power permits the resolution of more conformations for BPTI and cytochrome c, the resolved features are generally much broader than expected for a single rigid conformation. A major advantage of the new experimental configuration is the much gentler introduction of ions into the drift tube, so that the observed gas phase conformations appear to more closely reflect those present in solution. For example, it is possible to distinguish between the native state of cytochrome c and the methanol-denatured form on the basis of the ion mobility measurements; the mass spectra alone are not sensitive enough to detect this change. Thus this approach may provide a quick and sensitive tool for probing the solution phase conformations of biological molecules.
ISSN:0168-1176
1873-2801
DOI:10.1016/S0168-1176(97)00182-1