Ursodeoxycholic acid prevents hepatic cytochrome P450 isozyme reduction in rats with deoxycholic acid-induced liver injury

• Published in: Journal of hepatology 1999-08, Vol.31 (2), p.263-270
• Main Authors: Tanaka, Masami, Nakura, Hironori, Tateishi, Tomonori, Watanabe, Minoru, Nakaya, Sachiko, Kumai, Toshio, Kobayashi, Shinichi
• Format: Article
• Language: English
• Subjects: Animals; Bile acids; Biological and medical sciences; Cholagogues and Choleretics - pharmacology; Cholagogues and Choleretics - therapeutic use; Cholestasis - chemically induced; Cholestasis - drug therapy; Cholestasis - enzymology; Cytochrome P-450 Enzyme System - drug effects; Cytochrome P-450 Enzyme System - metabolism; Cytochrome P450; Deoxycholic acid; Deoxycholic Acid - toxicity; Digestive system; Drug-metabolizing system; Liver - enzymology; Liver - pathology; Liver injury; Male; Medical sciences; Pharmacology. Drug treatments; Rats; Rats, Sprague-Dawley; RNA, Messenger - drug effects; Ursodeoxycholic acid; Ursodeoxycholic Acid - pharmacology; Ursodeoxycholic Acid - therapeutic use
• ISSN: 0168-8278; 1600-0641
• DOI: 10.1016/S0168-8278(99)80223-2

Background/Aims: Hydrophobic bile acids, such as deoxycholic acid produce cholestatic liver injury. Ursodeoxycholic acid has been shown to be useful in the treatment of cholestatic liver disease. Methods: In this study, we investigated the effects of deoxycholic acid or ursodeoxycholic acid (1% of diet, for 14 days) and their combination (1% each) on expression of hepatic cytochrome P450 isozymes, their related enzyme activities and mRNA level in rats. Results: Adding 1% deoxycholic acid to chow caused a marked increase in serum total bilirubin (47-fold) and total bile acid (8-fold) concentrations and in alkaline phosphatase (2.5-fold, p