Lipolytic isoenzymes from Euphorbia latex

The activity and substrate specificity of latex lipases from Euphorbia species ( E. characias, E. wulfenii, E. pinea, E. myrsinites and E. dendroides) were investigated. High lipolytic activity was found only in E. characias and for the first time in E. wulfenii latex. For both species the lipolytic...

Full description

Saved in:
Bibliographic Details
Published in:Plant science (Limerick) 2003-09, Vol.165 (3), p.577-582
Main Authors: Palocci, Cleofe, Soro, Simonetta, Cernia, Enrico, Fiorillo, Fulvio, Belsito, Carmela M.A., Monacelli, Barbara, Delle Monache, Giuliano, Pasqua, Gabriella
Format: Article
Language:English
Subjects:
Biological and medical sciences
Enzymes
Euphorbia characias
Euphorbia wulfenii
Euphorbiaceae
Fundamental and applied biological sciences. Psychology
Latex
Metabolism
Plant lipases
Plant physiology and development
Terpenols
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The activity and substrate specificity of latex lipases from Euphorbia species ( E. characias, E. wulfenii, E. pinea, E. myrsinites and E. dendroides) were investigated. High lipolytic activity was found only in E. characias and for the first time in E. wulfenii latex. For both species the lipolytic activity on various triglycerides, and under different temperature and pH conditions, in both crude latex and in partially purified enzymes was quantified. Optimised extraction and purification methods permitted the recovery of the enzymatic fraction with high lipolytic activity. This fraction is probably constituted by a pool of different lipolytic enzymes. Finally, lipolytic activity was also measured for E. characias and E. wulfenii during vegetative and reproductive stages.
ISSN:0168-9452
1873-2259
DOI:10.1016/S0168-9452(03)00223-1