Isolation, characterization and expression analysis of a starch branching enzyme II cDNA from wheat

A full-length cDNA (2970 bp) encoding a starch branching enzyme II (SBEII; EC 2.4.1.18) in wheat ( Triticum aestivum L. cv Fielder) kernel was isolated from a cDNA library. The translated region of the cDNA predicted a 823 amino acid primary product with a molecular mass of 91.4 kDa. A 54 amino acid...

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Bibliographic Details
Published in:Plant science (Limerick) 1997-02, Vol.122 (2), p.153-163
Main Authors: Nair, Ramesh B., Båga, Monica, Scoles, Graham J., Kartha, Kutty K., Chibbar, Ravindra N.
Format: Article
Language:English
Subjects:
Biological and medical sciences
cDNA
Enzymes
Fundamental and applied biological sciences. Psychology
Gene expression
Metabolism
Plant physiology and development
Starch branching enzyme
Wheat ( Triticum aestivum L.)
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Summary:A full-length cDNA (2970 bp) encoding a starch branching enzyme II (SBEII; EC 2.4.1.18) in wheat ( Triticum aestivum L. cv Fielder) kernel was isolated from a cDNA library. The translated region of the cDNA predicted a 823 amino acid primary product with a molecular mass of 91.4 kDa. A 54 amino acid transit peptide was postulated to be cleaved from the pre-protein to give a 769 amino acid (85.4 kDa) mature polypeptide, which showed extensive sequence similarity to SBEII sequences characterized from maize, rice and pea. Expression of the isolated cDNA in a BE-deficient E. coli strain demonstrated that it encoded a functional BE. RNA analysis of Sbe2 gene expression during seed development revealed that Sbe2 mRNA levels were highest in young kernels (5–10 days post-anthesis) and declined as the kernels matured.
ISSN:0168-9452
1873-2259
DOI:10.1016/S0168-9452(96)04543-8