Impact of microspore developmental stage on induction and plant regeneration in rye anther culture

Rye anthers grouped into eight length classes, containing microspores from early uninucleate to binucleate developmental stage, were cultured to study the effect of microspore development on induction of embryogenesis and regeneration of green and albino plants. Three rye cultivars were monitored in...

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Bibliographic Details
Published in:Plant science (Limerick) 1998-12, Vol.139 (2), p.213-222
Main Authors: Immonen, Sirkka, Anttila, Hanna
Format: Article
Language:English
Subjects:
Anther and ovule culture, utilization for haploid production
ANTHER CULTURE
Biological and medical sciences
Biotechnology
CULTIVO DE ANTERAS
CULTURE D'ANTHERE
DESARROLLO EMBRIONARIO
DEVELOPPEMENT EMBRYONNAIRE
Embryoid quality
EMBRYONIC DEVELOPMENT
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Green plant regeneration
IN VITRO REGENERATION
Methods. Procedures. Technologies
Plant cells and fungal cells
Plant physiology and development
REGENERACION IN VITRO
Regeneration efficiency
REGENERATION IN VITRO
SECALE CEREALE
Tissue cultures, protoplasts
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Summary:Rye anthers grouped into eight length classes, containing microspores from early uninucleate to binucleate developmental stage, were cultured to study the effect of microspore development on induction of embryogenesis and regeneration of green and albino plants. Three rye cultivars were monitored in two tests. Callus and embryoid induction increased in all cases beyond first pollen mitosis. Embryo quality was influenced by microspore age, and the ability of embryoids to develop into plants decreased as induction rate increased. Microspore developmental stage did not influence the proportion of albino plants of all regenerants. These findings have consequencies for studies on regeneration and in determining the optimum microspore developmental stage for efficient regeneration of green rye plants.
ISSN:0168-9452
1873-2259
DOI:10.1016/S0168-9452(98)00191-5