Changes induced by NaCl in lipid content and composition, lipoxygenase, plasma membrane H +-ATPase and antioxidant enzyme activities of tomato ( Lycopersicon esculentum. Mill) calli

• Published in: Plant science (Limerick) 1999-05, Vol.143 (2), p.143-150
• Main Authors: Rodrı́guez-Rosales, M.P., Kerkeb, L., Bueno, P., Donaire, J.P.
• Format: Article
• Language: English
• Subjects: adenosinetriphosphatase; Agronomy. Soil science and plant productions; Antioxidant enzymes; ascorbic acid; Biological and medical sciences; biomass; callus; catalase; chemical constituents of plants; culture media; Economic plant physiology; enzyme activity; Fundamental and applied biological sciences. Psychology; glutathione peroxidase; H +-ATPase; hydrogen ions; ion transport; Lipids; Lipoxygenase; Lycopersicon esculentum; peroxidase; phosphatidylcholines; phosphatidylethanolamines; phospholipids; Plant physiology and development; respiration; salinity; Salt-tolerance; sodium chloride; Solanum lycopersicum var. lycopersicum; sterols; superoxide dismutase; Water and solutes. Absorption, translocation and permeability; Water relations, transpiration, stomata
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/S0168-9452(99)00046-1

Tomato ( Lycopersicon esculentum Mill. cv. Pera) calli tolerant to 50 mM NaCl were obtained by successive subcultures in NaCl supplemented medium. Salt-tolerant calli showed an increase of fresh and dry weight respective to control calli. When control and 50 mM NaCl-tolerant calli were stressed with 100 mM NaCl for 48 h, a decrease in respiration rate of 32 and 9%, respectively, was observed. Relative proportions of phospholipid fatty acids and free-sterol molecular species were the same in both control and NaCl tolerant calli. While the content of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) increased in salt-tolerant calli, the free sterol content was similar in both cases. A substantial increase of vanadate-sensitive ATP-dependent H + pumping activity without any modification in specific phosphohydrolytic activity and in passive H + conductance was detected in microsomes from salt-tolerant calli, which could be explained by an increased coupling between H + pumping and ATP hydrolysis. The higher lipoxygenase and antioxidant enzyme activities such as superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase and glutathione- S-transferase in 50 mM NaCl-tolerant calli as compared to controls also suggest that salt-tolerant calli has a high capacity of polyunsaturated fatty acid hydroperoxide formation and active oxygen species scavenging.