Optimization of media nitrogen and copper concentrations for regeneration of green plants from polyembryogenic cultures of barley ( Hordeum vulgare L.)

In recent years particle bombardment has become the most used method for gene transfer to barley ( Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryog...

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Bibliographic Details
Published in:Plant science (Limerick) 2000-02, Vol.151 (1), p.85-92
Main Authors: Nuutila, A.M, Hämäläinen, J, Mannonen, L
Format: Article
Language:English
Subjects:
Barley
Biological and medical sciences
Biotechnology
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Hordeum vulgare
In vitro propagation: entire plant regeneration from tissues and cell cultures
Mathematical models
Medium optimization
Methods. Procedures. Technologies
Plant cells and fungal cells
Polyembryogenesis
Regeneration
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Summary:In recent years particle bombardment has become the most used method for gene transfer to barley ( Hordeum vulgare L.). Transformation efficiency depends greatly on the ability of the target material to regenerate into green plants. In this work we improved the regeneration efficiency of polyembryogenic cultures of barley (cv. Kymppi) by optimizing the nitrogen and copper concentrations in the media.
ISSN:0168-9452
1873-2259
DOI:10.1016/S0168-9452(99)00202-2