An Active Sucrose Carrier (Scr1) that is Predominantly Expressed in the Seedling of Ricinus communis L

During the germination of Ricinus communis seedlings sucrose is released from the endosperm and taken up by the cotyledons for their growth and for export to the rapidly growing hypocotyl and roots. Both the mesophyll cells and the phloem of castor bean possess active sucrose carriers. We isolated o...

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Bibliographic Details
Published in:Journal of plant physiology 1996, Vol.147 (6), p.685-690
Main Authors: Weig, Alfons, Komor, Ewald
Format: Article
Language:English
Subjects:
ADN
AZUCAR
Biological and medical sciences
cDNA
Cell physiology
CELLS
CELLULE
CELULAS
COTILEDONES
COTYLEDON
COTYLEDONS
CRECIMIENTO
CROISSANCE
DNA
ENDOSPERM
ENDOSPERMA
ENDOSPERME
FEUILLE
FLOEMA
Fundamental and applied biological sciences. Psychology
GENE
GENES
Germination
GROWTH
Heterologous expression
HIPOCOTILOS
HOJAS
HYPOCOTYLE
HYPOCOTYLS
LEAVES
MALTOSA
MALTOSE
MESOFILO
MESOPHYLL
MESOPHYLLE
PEPTIDE
PEPTIDES
PEPTIDOS
Peptidsequenz
PHLOEM
PHLOEME
Plant physiology and development
PLANTULAS
PLANTULE
Plasma membrane and permeation
Quantitative PCR
RACINE
RAFFINOSE
RAFINOSA
RAICES
Ricinus
RICINUS COMMUNIS
ROOTS
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SACCHAROSE
Saccharosetraeger
SEEDLINGS
SUCRE
SUCROSA
SUCROSE
SUGAR
Sugar (sucrose) transport
Wunderbaum
Zuckertransport
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Description
Summary:During the germination of Ricinus communis seedlings sucrose is released from the endosperm and taken up by the cotyledons for their growth and for export to the rapidly growing hypocotyl and roots. Both the mesophyll cells and the phloem of castor bean possess active sucrose carriers. We isolated one of these carriers by heterologous screening with the spinach sucrose carrier cDNA for further investigation of sucrose transport at the cellular level. The deduced peptide sequence of this sucrose carrier (Scr1) has 12 putative membrane spanning domains and shares homology with other transporters of the major facilitator superfamily (MFS). When expressed in Saccharomyces cerevistae, Scr1 shows active sucrose transport that can be inhibited by PCMBS or CCCP. According to the pH-dependence the carrier seems related to SUC2 of Arabidopsis and Plantago, which are phloem-specific in these plants. It also shows a relatively high specificity for sucrose, compared with maltose or raffinose. Scr1 is expressed in equal amounts in all organs of a 6-day-old castor bean seedling and to a lesser extent in green sink and source leaves.
ISSN:0176-1617
1618-1328
DOI:10.1016/S0176-1617(11)81478-1