Somatic Embryogenesis and Plant Regeneration from Seedling Cultures of Tomato ( Lycopersicon esculentum Mill.)

Somatic embryogenesis was induced in several cultivars of tomato ( Lycopersicon esculentum Mill.) using a simple, one-step method. Seeds were germinated in the presence of 50 or 80 μmol · L -1 N 6-benzylaminopurine, and the resultant seedlings were maintained for six weeks on the same medium. Somati...

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Bibliographic Details
Published in:Journal of plant physiology 1995, Vol.147 (2), p.273-276
Main Authors: Gill, R., Malik, K.A., Sanago, M.H.M., Saxena, P.K.
Format: Article
Language:English
Subjects:
6-BENZILADENINA
6-BENZYLADENINE
Agronomy. Soil science and plant productions
AUXINAS
AUXINE
AUXINS
Biological and medical sciences
Biotechnology
CAL
CALLO
CALLUS
CULTIVO DE TEJIDOS
CULTURE DE TISSU
DESARROLLO EMBRIONARIO
DEVELOPPEMENT EMBRYONNAIRE
EMBRYONIC DEVELOPMENT
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Genetics and breeding of economic plants
Haploidy, in vitro culture applications, somatic hybrids
In vitro propagation: entire plant regeneration from tissues and cell cultures
LYCOPERSICON ESCULENTUM
MERISTEMAS
meristematisches Zentrum
MERISTEME
MERISTEMS
Methods. Procedures. Technologies
N 6-benzylaminopunne
NBenzylaminopurin
Plant breeding: fundamental aspects and methodology
Plant cells and fungal cells
PLANT GROWTH SUBSTANCES
Plant physiology and development
PLANTULAS
PLANTULE
Regeneration
Saemlingskultur
seedling culture
SEEDLINGS
somatic embryogenesis
somatische Embryogenese
SUBSTANCE DE CROISSANCE VEGETALE
SUSTANCIAS DE CRECIMIENTO VEGETAL
TISSUE CULTURE
Tissue cultures, protoplasts
TOMATE
Tomato
TOMATOES
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Description
Summary:Somatic embryogenesis was induced in several cultivars of tomato ( Lycopersicon esculentum Mill.) using a simple, one-step method. Seeds were germinated in the presence of 50 or 80 μmol · L -1 N 6-benzylaminopurine, and the resultant seedlings were maintained for six weeks on the same medium. Somatic embryogenesis occurred directly without an intervening callus proliferation phase from the meristematic centres formed in the subepidermal region of the nodular tissues developed at the basal ends of induced seedlings. Regenerated somatic embryos were successfully grown into whole plants.
ISSN:0176-1617
1618-1328
DOI:10.1016/S0176-1617(11)81518-X