Kinetics of 2,4-Dichlorophenoxyacetic Acid Content in an Auxin-Dependent Suspension Culture of Nicotiana tabacum Cells

• Published in: Journal of plant physiology 1995, Vol.147 (3), p.383-390
• Main Authors: Koens, K.B., Nicoloso, F.T., Van Vliet, Th. B., Harteveld, M., Boot, C.J.M., Van Iren, F., Mulder, P., Libbenga, K.R., Kijne, J.W.
• Format: Article
• Language: English
• Subjects: 2,4-D; 2,4-Dichlorphenoxiessigsaeure; AUXINAS; AUXINE; AUXINS; Biological and medical sciences; CELL CULTURE; cell division; cell suspensions; CELLS; CELLULE; CELULAS; CULTIVO DE CELULAS; CULTURE DE CELLULE; Fundamental and applied biological sciences. Psychology; HORMONAS; HORMONE; HORMONES; NICOTIANA TABACUM; Nicotiana tabacum L; Pflanzenhormon; PLANT GROWTH SUBSTANCES; Plant physiology and development; Proliferation; SUBSTANCE DE CROISSANCE VEGETALE; SUSTANCIAS DE CRECIMIENTO VEGETAL; Tissue cultures, protoplasts; Wuchsstoff; Zellsprossung; Zellsuspensionskultur
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/S0176-1617(11)82172-3

In a suspension culture of tobacco LT cells, grown on LS medium containing 2,4-dichlorophenoxyacetic acid as the sole plant growth regulator, kinetics of 2,4-D concentrations in the medium and in the cells have been studied in relation to cell proliferation. During increase of cell number, the mean volume per cell decreased until about 80h and then increased. Increase of cell number was accompanied by a stepwise decrease of 2,4-D concentration in the medium and by an increase of 2,4-D concentration in the biomaß. During the lag phase and the exponential phase, the total amount of free 2,4-D remained constant. Next, in the linear growth phase, cell division appeared to be limited by a shortage of 2,4-D. These observations fit in a structured model based on the aßumptions that 2,4-D uptake is mainly due to free diffusion and ion trap, and that limitation of cell division is dependent on a threshold concentration of 2,4-D in the medium.