An EPR study of wheat seeds by the use of nitroxide spin probes

• Published in: Journal of plant physiology 1997, Vol.151 (2), p.196-200
• Main Authors: Sünnetçioğlu, M. Maral, Dadayli, Dilek, Sungur, Riza, Bingöl, Gökçe
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Economic plant physiology; Embryo development. Germination; EPR; Fundamental and applied biological sciences. Psychology; Germination and dormancy; Growth and development; nitroxide spin probes; Plant physiology and development; simulation of EPR spectra; Wheat seed
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/S0176-1617(97)80152-6

The viability of a variety of wheat seeds was investigated by use of the EPR technique. Three different types of spin probes (4-Hydroxy-TEMPO (TANOL), 3-Carbamoyl-2,2,5,5-tetramethyl-3-pyrrolin-l-yloxy, 3-Carbamoyl-PROXYL) were utilized in examining the viability of embryo cells. The penetration of the spin probe across the membrane was observed via the change in signal intensity against time for dry embryos of the variety of wheat seeds soaked in aqueous solutions of spin probe and line broadening agent. From the analysis of the signal intensity-time curves (i.e. rehydration curves) it was observed that the two kinds having different genetic roots (Bezostaya, Kunduru) exhibit similar saturation behaviour whereas Gerek saturates much more easily. The rehydration curves for naturally aged seeds indicates that aging causes rapid saturation and an important decrease in the signal intensity. In order to get better insight on the effect of the signals from polar and nonpolar regions on the experimental spectra, these spectra were simulated by the use of the theoretical models developed.