Sucrose starvation causes a loss of immunologically detectable pyrophosphatase and V-ATPase in the tonoplast of suspension-cultured tobacco cells

• Published in: Journal of plant physiology 1998, Vol.152 (2), p.207-212
• Main Authors: Sikora, Adam, Hillmer, Stefan, Robinson, David G.
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Economic plant physiology; Fundamental and applied biological sciences. Psychology; Metabolism; Net assimilation, photosynthesis, carbon metabolism. Photorespiration, respiration, fermentation (anoxia, hypoxia); Nutrition. Photosynthesis. Respiration. Metabolism; Photosynthesis, respiration. Anabolism, catabolism; Plant physiology and development; Pyrophosphatase; Sucrose starvation; Tobacco; V-ATPase
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/S0176-1617(98)80134-X

The effect of sucrose starvation on logarithmically growing suspension cultures of tobacco BY-2 cells has been investigated by immunogold electron microscopy with antisera against pyrophosphatase (PPase) and V-ATPase. In normal cells PPase is located at the plasma and tonoplast membranes, whereas V-ATPase is restricted to the tonoplast. After 32 h of growth in the absence of exogenous sucrose, both PPase and V-ATPase polypeptides are no longer detectable in the tonoplast by this method. By contrast, plasma membrane-bound PPase appears to be unaffected by sucrose deficiency. These results are discussed in terms of a down-regulation of electrogenic pumps in the tonoplast.