The immune response of mice and cynomolgus monkeys to macaque mucin 1-mannan

Mice immunised with human epithelial mucin MUC1 coupled to oxidised mannan produce MUC1 specific MHC Class 1 restricted CD8 + cytotoxic T cells and are completely protected from the development of MUC1 + tumours; such therapy may be applicable to humans. In this light we describe pre-clinical studie...

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Bibliographic Details
Published in:Vaccine 2000-08, Vol.18 (28), p.3297-3309
Main Authors: Vaughan, Hilary A, Ho, Deborah W.M, Karanikas, Vaios, Sandrin, Mauro S, McKenzie, Ian F.C, Pietersz, Geoffrey A
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Antibodies
Base Sequence
Cross Reactions
CTL
Exons
Immunization
Lymphocyte Activation
Macaca mulatta
Mannans - immunology
Mice
Molecular Sequence Data
Mucin-1 - chemistry
Mucin-1 - genetics
Mucin-1 - immunology
Non-human primates
Polymorphism, Genetic
T-Lymphocytes, Cytotoxic - immunology
Tandem Repeat Sequences
Vaccination
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Summary:Mice immunised with human epithelial mucin MUC1 coupled to oxidised mannan produce MUC1 specific MHC Class 1 restricted CD8 + cytotoxic T cells and are completely protected from the development of MUC1 + tumours; such therapy may be applicable to humans. In this light we describe pre-clinical studies in cynomolgus monkeys ( Macaca fascicularis), to test the efficacy of mannan-MUC1 in higher primates. Monkey MUC1 genomic clones were isolated from a macaque library, peptides and fusion protein synthesised and mice and monkeys immunised with macaque MUC1-mannan. In mice CTL responses were induced (as has been found with human MUC1 mannan conjugates), but in contrast monkeys produced a humoral response, with no T cell proliferative, cytotoxic responses or CTLp found. In spite of the presence of anti-MUC1 auto-antibodies, there was no toxicity or induction of autoimmunity.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(00)00143-2