Immobilization of enzymes on lipid bilayers on a metal support allows study of the biophysical mechanisms of enzymatic reactions

We developed a glucose minisensor based on immobilization of the avidin-glucose oxidase (A-GOX) complex on biotinylated bilayer lipid membranes formed on a metal support (s-BLM). The immobilization of the A-GOX complex on a lipid bilayer was controlled by means of the electrostriction method which a...

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Bibliographic Details
Published in:Bioelectrochemistry and Bioenergetics 1996-12, Vol.41 (2), p.221-225
Main Authors: Hianik, Tibor, Šnejdárková, Maja, Passechnik, Victor I., Rehák, Marían
Format: Article
Language:English
Subjects:
Amperometric titration
Avidin
Bilayer lipid membranes
Electrostriction
Glucose oxidase
Solid support
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Summary:We developed a glucose minisensor based on immobilization of the avidin-glucose oxidase (A-GOX) complex on biotinylated bilayer lipid membranes formed on a metal support (s-BLM). The immobilization of the A-GOX complex on a lipid bilayer was controlled by means of the electrostriction method which allows us to determine the changes in membrane capacitance C, the elasticity modulus in the direction perpendicular to the s-BLM surface E⊥, and the trans-membrane potential ΔΦ m. The binding of the A-GOX complex to the s-BLM resulted in a decrease in C, increase in E⊥ and increase in ΔΦ m, suggesting that A-GOX represents a negatively charged complex which increases membrane ordering. Using the simple amperometric method we studied glucose oxidation by GOX and determined several important constants of the enzymatic reaction.
ISSN:0302-4598
DOI:10.1016/S0302-4598(96)05118-5