A multiplex-PCR/RFLP procedure for simultaneous CYP2E1, mEH and GSTM1 genotyping

Inter-individual variation in metabolism of environmental toxicants, which is attributed to genetic polymorphism, may be a major risk factor in determining who will develop adverse health effects. This priority research area is the focus of many laboratories, and new techniques need to be developed...

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Bibliographic Details
Published in:Cancer letters 1999-08, Vol.143 (1), p.51-56
Main Authors: Salama, Salama A., Sierra-Torres, Carlos H., Oh, Hye-Young, Hamada, Farid A., Au, William W.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
CYP2E1
Cytochrome P-450 CYP2E1 - genetics
DNA - analysis
DNA Primers - chemistry
Enzymes and enzyme inhibitors
Epoxide Hydrolases - genetics
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Genetic polymorphism
Genotype
Glutathione Transferase - genetics
GSTM1
Humans
mEH
Multiplex
Polymerase chain reaction
Polymerase Chain Reaction - methods
Polymorphism, Restriction Fragment Length
Restriction fragment length polymorphism
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Summary:Inter-individual variation in metabolism of environmental toxicants, which is attributed to genetic polymorphism, may be a major risk factor in determining who will develop adverse health effects. This priority research area is the focus of many laboratories, and new techniques need to be developed to enhance the efficiency in generating data. We have developed and validated a new multiplex-polymerase chain reaction (PCR), restriction fragment length polymorphism (RFLP) procedure for simultaneous genotyping of cytochrome P450 II E1 (CYP2E1), microsomal epoxide hydrolase (mEH), and glutathione S-transferase mu (GSTM1). Enzymes from these three polymorphic genes are involved with the phase I and II metabolism of a variety of environmental toxicants. Therefore, simultaneous characterization of these genes will not only reduce costs but will increase the efficiency of data collection, thereby contributing to health risk assessment efforts.
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(99)00192-5