Increased expression of cyclooxygenase-2 protein in rat lung tumors induced by N-nitrosobis(2-hydroxypropyl)amine

Expression of cyclooxygenase (COX)-2 protein in preneoplastic and neoplastic lung lesions induced by the administration of 2000 ppm of N-nitrosobis(2-hydroxypropyl)amine (BHP) in the drinking water to Wistar male rats, was examined immunohistochemically. The majority of alveolar/bronchiolar adenomas...

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Bibliographic Details
Published in:Cancer letters 2000-02, Vol.148 (2), p.145-152
Main Authors: KITAYAMA, W, DENDA, A, YOSHIDA, J.-I, SASAKI, Y, TAKAHAMA, M, MURAKAWA, K, TSUJIUCHI, T, TSUTSUMI, M, KONISHI, Y
Format: Article
Language:English
Subjects:
Adenocarcinoma, Bronchiolo-Alveolar - chemically induced
Adenocarcinoma, Bronchiolo-Alveolar - enzymology
Animals
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Carcinogens - toxicity
Carcinoma, Non-Small-Cell Lung - chemically induced
Carcinoma, Non-Small-Cell Lung - enzymology
Chemical agents
Cyclooxygenase 1
Cyclooxygenase 2
Enzyme Induction - drug effects
Growth Disorders - chemically induced
Growth Disorders - enzymology
Isoenzymes - biosynthesis
Isoenzymes - metabolism
Lung Neoplasms - chemically induced
Lung Neoplasms - enzymology
Male
Medical sciences
Membrane Proteins
Mice
Nitrosamines - toxicity
Precancerous Conditions - chemically induced
Precancerous Conditions - enzymology
Prostaglandin-Endoperoxide Synthases - biosynthesis
Prostaglandin-Endoperoxide Synthases - metabolism
Rats
Rats, Wistar
Tumors
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Summary:Expression of cyclooxygenase (COX)-2 protein in preneoplastic and neoplastic lung lesions induced by the administration of 2000 ppm of N-nitrosobis(2-hydroxypropyl)amine (BHP) in the drinking water to Wistar male rats, was examined immunohistochemically. The majority of alveolar/bronchiolar adenomas (ADs) and all adenocarcinomas (ADCs) examined, stained positive or strongly positive for COX-2. In contrast, only a minority of alveolar/bronchiolar hyperplasias demonstrated immunoreactivity and half of the squamous cell carcinomas examined, were only weakly positive. Western blotting analysis also revealed expression of COX-2 protein in the resected ADs and ADCs. These results clearly indicate up-regulated expression of COX-2 in lung neoplastic lesions, particularly ADs and ADCs, induced by BHP in rats.
ISSN:0304-3835
1872-7980
DOI:10.1016/s0304-3835(99)00325-0