Modulation of BmK AS, a scorpion neurotoxic polypeptide, on voltage-gated Na + channels in B104 neuronal cell line

The modulation of BmK AS, a neurotoxic polypeptide from scorpion Buthus martensi Karsch, on the voltage-gated Na + channels has been investigated in the B104 neuroblastoma cell line by whole-cell patch clamping. It was found that the whole Na + currents and tetrodotoxin-resistant Na + current were d...

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Published in:Neuroscience letters 2003-04, Vol.340 (2), p.123-126
Main Authors: Tan, Zhi-Yong, Chen, Jin, Shun, Hai-Ying, Feng, Xing-Hua, Ji, Yong-Hua
Format: Article
Language:English
Subjects:
Animals
B104
Biological and medical sciences
BmK AS
Cell membranes. Ionic channels. Membrane pores
Cell structures and functions
Dose-Response Relationship, Drug
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Neurons - drug effects
Neurons - physiology
Peptides - toxicity
Rats
Scorpion neurotoxin
Scorpion Venoms - toxicity
Sodium Channels - physiology
Tetrodotoxin - pharmacology
Tumor Cells, Cultured
Voltage-gated Na + channel
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Summary:The modulation of BmK AS, a neurotoxic polypeptide from scorpion Buthus martensi Karsch, on the voltage-gated Na + channels has been investigated in the B104 neuroblastoma cell line by whole-cell patch clamping. It was found that the whole Na + currents and tetrodotoxin-resistant Na + current were depressed in a biphasic manner and both steady-state activation and inactivation curves were shifted toward the hyperpolarizing direction in the presence of BmK AS. The results suggested that BmK AS could inhibit both tetrodotoxin-sensitive and -resistant Na + currents via modulating the gating mechanism of the voltage-gated Na + channels in B104 cells.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(03)00094-6