Isolation and characterization of DM40 and DM43, two snake venom metalloproteinase inhibitors from Didelphis marsupialis serum

• Published in: Biochimica et biophysica acta 2000-05, Vol.1474 (3), p.309-320
• Main Authors: Neves-Ferreira, Ana G.C, Cardinale, Norma, Rocha, Surza L.G, Perales, Jonas, Domont, Gilberto B
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Amino Acids - analysis; Animals; Antivenins - chemistry; Antivenins - isolation & purification; Blood Proteins - chemistry; Blood Proteins - isolation & purification; Bothrops jararaca; Bothrops jararaca Venom; Caseins - antagonists & inhibitors; Chromatography, Gel; Crotalid Venoms - chemistry; Crotalid Venoms - enzymology; Didelphis marsupialis; Electrophoresis, Polyacrylamide Gel; Glycosylation; Inhibitor; Isoelectric Point; Mass Spectrometry; Metalloendopeptidases - chemistry; Metalloproteinase; Molecular Sequence Data; Molecular Weight; Opossums - blood; Opossums - metabolism; Proteins - chemistry; Proteins - isolation & purification; Sequence Homology, Amino Acid; Snake venom
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/S0304-4165(00)00022-2

From Didelphis marsupialis serum, two antihemorrhagic proteins were isolated by DEAE-Sephacel, Phenyl-Sepharose and Superdex 200 and characterized. Their masses by mass spectrometry were 40 318 AMU for DM40 and 42 373 and 43 010 AMU for DM43, indicating the presence of isoforms for the last. Molecular masses of 44.8 and 47.3 were obtained by SDS–PAGE, respectively for DM40 and DM43. Both inhibitors showed isoelectric points lower than 3.5 and glycosylation percentages varying from 20.5 to 29.0%, as estimated by chemical deglycosylation and amino acid analysis. N-terminal sequences of the first 17 residues of DM40 and DM43 were identical except for the exchange of R9 for P9. Both were homologous to oprin, a similar inhibitor from Didelphis virginiana serum. No evidence of complex formation between DM40 and DM43 was observed either by native PAGE or gel filtration chromatography. In addition to the antihemorrhagic activity, DM40 and DM43 inhibited the hydrolysis of casein, fibrinogen and fibronectin by Bothrops jararaca venom. DM43 also showed antilethal, antiedematogenic and antihyperalgesic activities. None of the inhibitors showed enzymatic activity on casein. Both proteins formed stable complexes with jararhagin and inhibited its hemorrhagic effect as well as the enzymatic activity of this toxin on fluorogenic substrate.