Acetylphenylhydrazine induced haemoglobin oxidation in erythrocytes studied by Mössbauer spectroscopy

The oxidative action of acetylphenylhydrazine (APH) on red blood cells obtained from healthy donors and from patients with breast cancer has been investigated by Mössbauer spectroscopy. Whole blood was incubated with APH for different time periods and the Mössbauer spectra of the packed red cells we...

Full description

Saved in:
Bibliographic Details
Published in:Biochimica et biophysica acta 2001-11, Vol.1568 (1), p.99-104
Main Authors: Croci, S, Pedrazzi, G, Passeri, G, Piccolo, P, Ortalli, I
Format: Article
Language:English
Subjects:
Acetylphenylhydrazine
Breast cancer
Breast Neoplasms - blood
Erythrocyte
Erythrocytes - drug effects
Erythrocytes - metabolism
Female
Hemin - analysis
Hemoglobin oxidation
Hemoglobins - analysis
Hemoglobins - metabolism
Humans
Methemoglobin - analysis
Mössbauer spectroscopy
Oxidants
Oxidation-Reduction
Oxyhemoglobins - analysis
Phenylhydrazines
Spectroscopy, Mossbauer
Time Factors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The oxidative action of acetylphenylhydrazine (APH) on red blood cells obtained from healthy donors and from patients with breast cancer has been investigated by Mössbauer spectroscopy. Whole blood was incubated with APH for different time periods and the Mössbauer spectra of the packed red cells were recorded and compared. The evolution with time of the oxidation products has been followed. The largest difference in red cells analysis between healthy persons and patients was found after about 50 min of treatment where Mössbauer spectra of patient samples show a much broader spectral pattern due to an advanced haemoglobin oxidation.
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/S0304-4165(01)00205-7