Cloning and nucleotide sequence analysis of the Streptococcus sobrinus gtfU gene that produces a highly branched water-soluble glucan

Streptococcus sobrinus has four gtf genes, gtfI, gtfS, gtfT, and gtfU, on the chromosome. These genes correspond respectively to the enzymes GTF-I, GTF-S 1, GTF-S 2, and GTF-S 3. An Escherichia coli MD66 clone that contained the S. sobrinus gtfU gene was characterized. Immunological properties showe...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2002-02, Vol.1570 (1), p.75-79
Main Authors: Hanada, Nobuhiro, Fukushima, Kazuo, Nomura, Yoshiaki, Senpuku, Hidenobu, Hayakawa, Mitsuo, Mukasa, Hidehiko, Shiroza, Teruaki, Abiko, Yoshimitsu
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Binding Sites
Cloning, Molecular
Escherichia coli - genetics
Escherichia coli - metabolism
Genes, Bacterial
Glucans - biosynthesis
Glucans - chemistry
Glucosyltransferase
Glucosyltransferases - biosynthesis
Glucosyltransferases - chemistry
Glucosyltransferases - metabolism
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Streptococcus sobrinus - enzymology
Streptococcus sobrinus - genetics
Transfection
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Summary:Streptococcus sobrinus has four gtf genes, gtfI, gtfS, gtfT, and gtfU, on the chromosome. These genes correspond respectively to the enzymes GTF-I, GTF-S 1, GTF-S 2, and GTF-S 3. An Escherichia coli MD66 clone that contained the S. sobrinus gtfU gene was characterized. Immunological properties showed that the protein produced by the E. coli MD66 clone was similar to S. sobrinus GTF-S 1. Biological properties and a linkage analysis of the glucans by 13C NMR spectrometry revealed that the protein produced by the E. coli MD66 clone was GTF-S 1.
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/S0304-4165(01)00240-9