Evaluation of progesterone receptor expression in eosinophils using real-time quantitative PCR

Progesterone has been shown in many instances to have immune-suppressant activities. Most of these activities have been investigated in the light of general immune suppression or with a focus on lymphocytes. However, many clinical and in vitro studies have shown that progesterone also has a suppress...

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Bibliographic Details
Published in:Biochimica et biophysica acta 2002-07, Vol.1571 (3), p.167-172
Main Authors: Aerts, Joeri L.E, Christiaens, Marie-Rose, Vandekerckhove, Philippe
Format: Article
Language:English
Subjects:
Adult
Breast Neoplasms - chemistry
Breast Neoplasms - metabolism
Breast Neoplasms - pathology
Carcinoma, Ductal, Breast - chemistry
Carcinoma, Ductal, Breast - metabolism
Carcinoma, Ductal, Breast - pathology
Eosinophil
Eosinophils - chemistry
Eosinophils - cytology
Eosinophils - metabolism
Female
Humans
Immune-suppression
Immunohistochemistry
Male
Middle Aged
Neutrophils - chemistry
Neutrophils - cytology
Neutrophils - metabolism
Polymerase Chain Reaction - methods
Progesterone receptor
Quantitative PCR
Receptors, Progesterone - analysis
Receptors, Progesterone - biosynthesis
Receptors, Progesterone - genetics
Reference Values
Reproducibility of Results
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
Sensitivity and Specificity
Tumor Cells, Cultured
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Summary:Progesterone has been shown in many instances to have immune-suppressant activities. Most of these activities have been investigated in the light of general immune suppression or with a focus on lymphocytes. However, many clinical and in vitro studies have shown that progesterone also has a suppressive effect on eosinophilia. This effect so far has not been thoroughly investigated. The purpose of this study was to evaluate whether the effect is mediated via the classical progesterone receptor (PR). We developed a new real-time quantitative PCR (RQ-PCR) for the analysis and quantification of expression of the classical PR. The test was first validated both on breast cancer cell lines and on breast cancer biopsies. Subsequently, when using eosinophils isolated from peripheral blood of healthy volunteers, we could not find evidence for the expression of PR. These data suggest that the effects of progesterone on eosinophils are not mediated by the classical PR.
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/S0304-4165(02)00192-7