A new mechanism of action of fluoride on streptococci

• Published in: Biochimica et biophysica acta 1999-08, Vol.1428 (2), p.415-423
• Main Authors: Cox, S.D., Lassiter, M.O., Miller, Brian S., Doyle, R.J.
• Format: Article
• Language: English
• Subjects: Bacterial Proteins; Culture Media; Electrophoresis, Gel, Two-Dimensional; Fluoride; Glucan; Lectin; Lectins - analysis; Lectins - deficiency; Sodium Fluoride - pharmacology; Streptococci; Streptococcus sobrinus - chemistry; Streptococcus sobrinus - drug effects; Streptococcus sobrinus - growth & development; Time Factors
• ISSN: 0304-4165; 0006-3002; 1872-8006
• DOI: 10.1016/S0304-4165(99)00052-5

Addition of fluoride to the growth medium of Streptococcus sobrinus resulted in a loss of glucan-binding lectin activity. Upon removal of fluoride, the bacteria regained their ability to bind glucan in about one generation. Chloramphenicol prevented recovery of ability to produce the lectin, showing the requirement for protein synthesis. Fluoride also caused a significant reduction in the tendency of the streptococci to form chains of cells, although the spent medium from fluoride-containing growth media did not dechain control cells. The fluoride thus does not activate autolytic enzymes. Importantly, 2-D electrophoresis and SDS-PAGE revealed several proteins were synthesized in the presence of fluoride that were not synthesized in its absence. It seems possible that fluoride places a stress on the bacteria, causing the synthesis of proteins that may play a role in protecting the cells against the stress. Numerous stress proteins are known for bacteria, including those resulting from heat, enzymes and osmotic shocks. The ability of fluoride to cause loss of glucan-binding may be related to its reported beneficial effects on oral health.