Platelet aggregation may not be a prerequisite for collagen-stimulated platelet generation of nitric oxide

By determining the sum of the supernatant concentrations of nitrite and nitrate the stimulated generation of nitric oxide (NO) by human washed platelets induced by a range of fibrillar collagen concentrations (0.0156–25 μg ml −1) was investigated. Platelet serotonin (5-hydroxytryptamine, 5-HT) efflu...

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Bibliographic Details
Published in:Biochimica et biophysica acta 1999-12, Vol.1473 (2), p.286-292
Main Authors: Smith, Christopher C.T, Stanyer, Lee, Cooper, Michael B, Betteridge, D.John
Format: Article
Language:English
Subjects:
Aggregation
Blood Platelets - drug effects
Blood Platelets - metabolism
Collagen - pharmacology
Dose-Response Relationship, Drug
Endothelium, Vascular - drug effects
Endothelium, Vascular - metabolism
Enzyme Inhibitors - pharmacology
Humans
NG-Nitroarginine Methyl Ester - pharmacology
Nitrate
Nitric oxide
Nitric Oxide - metabolism
Nitrite
Platelet
Platelet Aggregation - drug effects
Sensitivity and Specificity
Serotonin
Serotonin - metabolism
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Summary:By determining the sum of the supernatant concentrations of nitrite and nitrate the stimulated generation of nitric oxide (NO) by human washed platelets induced by a range of fibrillar collagen concentrations (0.0156–25 μg ml −1) was investigated. Platelet serotonin (5-hydroxytryptamine, 5-HT) efflux and platelet aggregation were also measured. Under resting conditions (0 μg ml −1 collagen) platelet NO release was equivalent to 1.06±0.17 nmol per 10 8 platelets. Maximal NO release, equivalent to 2.1±0.37 nmol per 10 8 platelets, was observed with only 0.0625 μg ml −1 collagen ( P
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/S0304-4165(99)00202-0