The viability of asparagus pollen after storage at low temperatures

To assess the feasibility of conserving viable pollen of garden asparagus for breeding purposes using a simple and reliable technique, pollen samples from five plants (genotypes) of cv. ‘UC 157 F 2’ were stored in gelatin capsules at 4 °C for eight periods (0–140 days). Viability was estimated at th...

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Bibliographic Details
Published in:Scientia horticulturae 1996-11, Vol.67 (1), p.101-104
Main Authors: Marcellán, O.N., Camadro, E.L.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
ALMACENAMIENTO
AMELIORATION DES PLANTES
ASPARAGUS OFFICINALIS
Biological and medical sciences
COLD
FITOMEJORAMIENTO
FRIO
FROID
Fundamental and applied biological sciences. Psychology
Genetics and breeding of economic plants
GENOTIPOS
GENOTYPE
GENOTYPES
GERMINACION
GERMINATION
In vitro germination
PLANT BREEDING
POLEN
POLLEN
Pollen viability
Seeds and other planting stocks
STOCKAGE
STORAGE
VIABILIDAD
VIABILITE
VIABILITY
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Summary:To assess the feasibility of conserving viable pollen of garden asparagus for breeding purposes using a simple and reliable technique, pollen samples from five plants (genotypes) of cv. ‘UC 157 F 2’ were stored in gelatin capsules at 4 °C for eight periods (0–140 days). Viability was estimated at the end of each period by (a) staining and (b) in vitro germination. Staining did not reveal changes in viability during storage. However, the percentage of in vitro germination diminished from relatively high values (around 70%) during the first 14 days of storage to very low ones (around 5%) at 140 days, with the rate of decline dependent on genotype. It is, therefore, feasible to use pollen stored at low temperatures during most of the flowering period, but for storage periods over 1 month it is advisable to check the viability by in vitro germination before pollination.
ISSN:0304-4238
1879-1018
DOI:10.1016/S0304-4238(96)00949-1