20-Hydroxyecdysone 2-β- d-glucopyranoside from the seeds of Xerophyllum tenax

Bioassay/RIA-directed HPLC purification of a MeOH extract of the seeds of Xerophyllum tenax yielded 20-hydroxyecdysone and 20-hydroxyecdysone 2-β- d-glucopyranoside, the latter being a novel natural product. The structures were elucidated primarily on the basis of 1D and 2D NMR spectroscopic evidenc...

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Bibliographic Details
Published in:Biochemical systematics and ecology 1997-04, Vol.25 (3), p.255-261
Main Authors: Alison, Brenda, Whiting, Pensri, Sarker, Satyajit D., Dinan, Laurence, Underwood, Eric, Šik, Vladimir, Rees, Huw H.
Format: Article
Language:English
Subjects:
chemotaxonomy
Drosophila melanogaster, bioassay
ecdysteroid
Liliaceae
Melanthiaceae
seeds
steroid
Xerophyllum tenax
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Summary:Bioassay/RIA-directed HPLC purification of a MeOH extract of the seeds of Xerophyllum tenax yielded 20-hydroxyecdysone and 20-hydroxyecdysone 2-β- d-glucopyranoside, the latter being a novel natural product. The structures were elucidated primarily on the basis of 1D and 2D NMR spectroscopic evidence. The ecdysteroid agonist activity of these isolates was determined on the B II tumorous blood cell line of Drosophila melanogaster. ED 50 values for 20-hydroxyecdysone and 20-hydroxyecdysone 2-β- d-glucopyra-noside were found to be 8.0 × 10 −9M and 1.3 × 10 −4M, respectively. The significance of the presence of ecdysteroid conjugates in plants is discussed.
ISSN:0305-1978
1873-2925
DOI:10.1016/S0305-1978(97)00003-3