A simple experiment demonstrating the allosteric regulation of yeast pyruvate kinase

A simple experiment in which the regulatory properties of yeast pyruvate kinase are determined. Students do a partial purification using PEG precipitation and find that fructose-1,6-bisphosphate activates the enzyme. ATP and alanine negatively modulate its catalytic activity. The entire experiment c...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical education 1998, Vol.26 (1), p.73-76
Main Authors: Taber, Richard L., Campbell, Angela, Spencer, Scott
Format: Article
Language:English
Subjects:
Biochemistry
Chemical Reactions
College Curriculum
Higher Education
Laboratory Procedures
Science Activities
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A simple experiment in which the regulatory properties of yeast pyruvate kinase are determined. Students do a partial purification using PEG precipitation and find that fructose-1,6-bisphosphate activates the enzyme. ATP and alanine negatively modulate its catalytic activity. The entire experiment can be done in one laboratory period using simple equipment.
ISSN:0307-4412
1879-1468
DOI:10.1016/S0307-4412(97)00117-9