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Analysis of green tea catechins: comparative study between HPLC and HPCE

A comparison between a borate–phosphate–SDS based MEKC and an RP-HPLC method for the separation of seven tea catechins and gallic acid in a green tea extract is here proposed. Under optimised conditions, HPCE offered several advantages respect to time of analysis (compounds were separated within 4.5...

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Bibliographic Details
Published in:Food chemistry 2003-06, Vol.81 (4), p.631-638
Main Authors: Bonoli, Matteo, Pelillo, Marco, Toschi, Tullia Gallina, Lercker, Giovanni
Format: Article
Language:English
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Summary:A comparison between a borate–phosphate–SDS based MEKC and an RP-HPLC method for the separation of seven tea catechins and gallic acid in a green tea extract is here proposed. Under optimised conditions, HPCE offered several advantages respect to time of analysis (compounds were separated within 4.5 min), sensitivity (HPCE LODs were about 20–100 times lower than HPLC ones) and solvent consumption. HPCE displayed excellent migration time repeatability (RSD% on MT
ISSN:0308-8146
1873-7072
DOI:10.1016/S0308-8146(02)00565-4