Preparation of (–)-epigallocatechin gallate from commercial green tea by caffeine precipitation and solvent partition

A method has been developed which enables the easy and inexpensive preparation of gram quantities of (–)-epigallocatechin gallate from green tea ( Camellia sinensis). A decaffeinated aqueous brew of commercial green tea is treated with caffeine (30 m m). The precipitate is redissolved after decaffei...

Full description

Saved in:
Bibliographic Details
Published in:Food chemistry 1998, Vol.61 (1), p.81-87
Main Authors: Copeland, E.L, Clifford, M.N, Williams, C.M
Format: Article
Language:English
Subjects:
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A method has been developed which enables the easy and inexpensive preparation of gram quantities of (–)-epigallocatechin gallate from green tea ( Camellia sinensis). A decaffeinated aqueous brew of commercial green tea is treated with caffeine (30 m m). The precipitate is redissolved after decaffeination with chloroform and further purified by solvent partition with ethyl hexanoate and propyl acetate. Commercial leaf (25 g) yields 400 mg (–)-epigallocatechin gallate at better than 80% purity, as judged by reversed phase HPLC.
ISSN:0308-8146
1873-7072
DOI:10.1016/S0308-8146(97)00111-8