Separation by solid phase extraction and quantification by reverse phase HPLC of sulforaphane in broccoli

The separation and quantification of sulforaphane [1-isothiocyanato-4-(methyl-sulfinyl)-butane] from the florets, stalks and leaves of broccoli is described. The procedure uses solvent extraction, followed by purification of extracts using solid phase extraction (SPE) and reverse phase HPLC analysis...

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Bibliographic Details
Published in:Food chemistry 1998-11, Vol.63 (3), p.417-421
Main Authors: Bertelli, Davide, Plessi, Maria, Braghiroli, Daniela, Monzani, Agar
Format: Article
Language:English
Subjects:
Biological and medical sciences
General pharmacology
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
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Summary:The separation and quantification of sulforaphane [1-isothiocyanato-4-(methyl-sulfinyl)-butane] from the florets, stalks and leaves of broccoli is described. The procedure uses solvent extraction, followed by purification of extracts using solid phase extraction (SPE) and reverse phase HPLC analysis. The HPLC method is compared with a spectrophotometric assay. To obtain information about the usefulness of acid hydrolysis of glucosinolates, the florets were left to autolyze at room temperature or were treated with concentrated hydrochloric acid, then analysed. The method proves reliable and reproducible as regards both SPE purification and chromatographic determination. Quantities of sulforaphane were found in the florets, stalks and leaves. The highest content of sulforaphane (110 μgg −1) was found in the leaves.
ISSN:0308-8146
1873-7072
DOI:10.1016/S0308-8146(98)00052-1