Determination of biotin in foods by high-performance liquid chromatography with post-column derivatization and fluorimetric detection

A method to determine the content of biotin in various foods by reverse phase liquid chromatography is proposed and includes extraction of the vitamers ( d-biotin and d-biocytin) by enzymatic hydrolysis (papain) and takadiastase if necessary, post-column derivatization by avidin-FITC (fluorescein 5-...

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Bibliographic Details
Published in:Food chemistry 1999-05, Vol.65 (2), p.253-258
Main Authors: Lahély, S, Ndaw, S, Arella, F, Hasselmann, C
Format: Article
Language:English
Subjects:
Biological and medical sciences
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Methods of analysis, processing and quality control, regulation, standards
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Summary:A method to determine the content of biotin in various foods by reverse phase liquid chromatography is proposed and includes extraction of the vitamers ( d-biotin and d-biocytin) by enzymatic hydrolysis (papain) and takadiastase if necessary, post-column derivatization by avidin-FITC (fluorescein 5-isothiocyanate) and fluorimetric detection of the complex obtained. Enzymatic hydrolysis is preferred to sulfuric acid hydrolysis because it does not induce any degradation of the vitamin and allows a good estimation of the bioavailable forms. The proposed method has a good recovery rate (90–106%), a satisfactory repeatability (coefficient of variation less than 7%) and a very low detection limit (0.005 μg g −1).
ISSN:0308-8146
1873-7072
DOI:10.1016/S0308-8146(98)00185-X