Detection of the 20-kDa virulence-associated antigen of Rhodococcus equi in malakoplakia-like lesion in pleural tissue obtained from an AIDS patient

• Published in: Pathology, research and practice research and practice, 2000, Vol.196 (5), p.321-327
• Main Authors: Caterino-de-Araujo, Adele, de los Santos-Fortuna, Elizabeth, Zandoná-Meleiro, Mônica Cristina, Calore, Edenilson Eduardo, Calore, Nilda Maria Perez
• Format: Article
• Language: English
• Subjects: Actinomycetales Infections - immunology; Actinomycetales Infections - microbiology; Actinomycetales Infections - pathology; Adult; AIDS; AIDS-Related Opportunistic Infections - immunology; AIDS-Related Opportunistic Infections - microbiology; AIDS-Related Opportunistic Infections - pathology; Animals; Antibodies, Monoclonal; Antigens, Bacterial - analysis; Humans; Immunoenzyme Techniques; Immunohistochemistry; Macrophages; Macrophages, Alveolar - microbiology; Macrophages, Alveolar - pathology; Malacoplakia - immunology; Malacoplakia - microbiology; Malacoplakia - pathology; Malakoplakia; Male; Mice; Molecular Weight; Pleural Diseases - immunology; Pleural Diseases - microbiology; Pleural Diseases - pathology; Rhodococcus equi; Rhodococcus equi - immunology; Rhodococcus equi - pathogenicity; Substance Abuse, Intravenous; Virulence - immunology; Virulence-associated antigen of 20-kDa
• ISSN: 0344-0338; 1618-0631
• DOI: 10.1016/S0344-0338(00)80062-X

A malakoplakia-like lesion was detected in a pleural biopsy from an AIDS patient presenting clinical and radiologic features of pneumonia. Cultures of bronchoalveolar lavage and pleural fluid evidenced Rhodococcus equi as the causative agent of pleuro-pulmonary infection. Immunochemical characterization of the R. equi isolate showed the presence of a strain similar to the ATCC 33704 reference strain presenting the capsular antigen of serotype 4, and the intermediate virulence-associated antigen of 20-kDa. Histopathology of the patient's pleural biopsy showed plaques of macrophages interspersed with lymphocytes, and intracytoplasmic cocci and bacilli in macrophages, which were variably acid-fast positive. Immunohistochemistry of cocci, bacilli and their degradation products resulted strongly positive when stained with a mouse monoclonal antibody (MAb) produced against the 20-kDa antigen. This finding could have important implications for the pathogenicity of R. equi for human beings, since we do not know yet all the factors involved in the formation of malakoplakia. Indeed, the results obtained in the present study, taken together with the results obtained for pigs inoculated with R. equi strains of intermediate virulence (Madarame et al. 1998), raise the possibility that most strains presenting the 20-kDa antigen may be capable of inducing malakoplakia. If this hyphothesis is confirmed by immunohistochemical analysis of human pulmonary malakoplakia cases due to R. equi, the detection of this antigen may be extremely helpful in the diagnosis and treatment of such patients. This is the first report of R. equi infection in human beings that suggests a relationship between pleural malakoplakia and the virulence-associated antigen of 20-kDa.